Quality Standard：【产品介绍】Actins are highly conserved
proteins ubiquitously expressed in all eukaryotic
cells. They comprise, along with microtubules, a
major component of the cytoskeleton. Actin has been found
to be expressed in at least six isomeric forms. It
is expressed in heart and skeletal striated muscle
tissue, and in certain smooth muscle tissues,
regulating contractile potentials for these cells.
It is also expressed in the cytoplasm of non-muscle
cells, functioning to control cell structure and
motility (1). Polymerization of globular actin
(G-actin) leads to a structural filament (F-actin),
whose formation requires the Arp2/3 complex (2). It
has been reported that actin is hyperphosphorylated
in primary breast tumors (3). It has also been shown
that actin dysfunction leads to certain types of
heart failure (4). Smooth muscle alpha actin is of
further interest because it is one of a few genes
whose expression is relatively restricted to vascular
smooth muscle cells. Furthermore, expression of
smooth muscle alpha actin is regulated by hormones and
cell proliferation, and is altered by
pathological conditions including oncogenic
transformation and atherosclerosis (5).
Recommended Applications
IHC, ICC, FC
Applications and Recommended Dilution Factors
IHC: 1:1000 - 2500
ICC: 1:250 - 500
FC: 1:10 - 100
Species Reactivity
Human, Mouse, Rat
Products Data
Immunohistochemical analysis of paraffin-embedded
human heart tissue using anti-Alpha-Actin
(Smooth Muscle) RabMAb (cat.# 5264-1).
Immunohistochemical analysis of paraffin-embedded
human ovary tissue using anti-Alpha-Actin
(Smooth Muscle) RabMAb (cat.# 5264-1).
Specificity
A synthetic peptide corresponding to residues on
the N-terminus in human Alpha-Actin (Smooth Muscle) was
used as an immunogen.
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