Rabbit Monoclonal Antibody

Molecular Formula：

Molecular Weight：

Alias：
BRAF antibody, B-RAF1 antibody, BRAF1 antibody, FLJ95109 antibody, MGC126806 antibody, MGC138284 antibody, NS7 antibody, RAFB1 antibody, Serine/threonine-protein kinase B-raf antibody, Proto-oncogene B-Raf antibody, p94 antibody, v-Raf murine sarcoma vira

Product order：

Name	Specifications	Packing	Price	Delivery
Rabbit Monoclonal Antibody		100ul	Inquiry	现货

Character：
【反应特异性】Human , Mouse , Rat
【克隆 ID】EPR2207
【用途】WB , IHC
【抗原分子量】94kDa

Quality Standard：
【产品介绍】B-Raf, also know as c-Rmil, is
a Serine/Threonine protein kinase member of the Raf
family which includes Raf-1 and A-Raf. B-Raf is involved
in the transduction of mitogenic signals from the
cell membrane to the nucleus (1). Composed of
three conserved region (CR1, CR2, CR3) B-Raf is
expressed primarily in the brain and in the nervous
system (2). It has been observed that MAPK is activated
by B-Raf in response to nerve growth factor (3). More
than 60% of malignant melanomas were found to contain
a specific mutation, B-Raf (V599E) the product of
which possesses constitutive kinase activity (4).
Mutations in B-Raf have also been identified in lung
cancer and non-Hodgkin lymphoma.

Recommended Applications

WB, IHC
Applications and Recommended Dilution Factors

WB: 1:1,000 - 10,000
IHC: 1:100 - 250
Species Reactivity *

Human, Mouse, Rat

*Species cross-reactivity is based on WB analysis.

Products Data
Western blot analysis on PC-12 cell lysates
using anti-Phospho-B-Raf (pS729) RabMAb
(cat. #5170-1). Cells were either (A) untreated (B)
treated with Lambda Phosphatase.

Specificity

A phospho specific peptide corresponding to
residues surrounding serine 729 of human B-Raf was used
as an immunogen. This antibody detects B-Raf
phosphorylated on serine 729.

Storage：
Storage Condition and Buffer

Store at -20 °C. Antibody buffer, 0.01% sodium azide,
50% Glycerol and 0.05% BSA. Stable for 12 months from date
of receipt