Quality Standard：【产品介绍】Focal adhesion kinase (FAK) is a non-recept
o r protein-tyrosine kinase implicated in signaling
pathways involved in cell motility, proliferation
a n d apoptosis (1). FAK is composed of a central
catalytic domain flanked by large N- a n d
C-terminal regions. FAK is activated by phosph o r
ylation at tyrosine 397 in response to integrin
clustering which can be induced by cell adhesion
o r antibody cross-linking o r via G-protein-coupled
recept o r (GPCR) occupancy by lig a n d s such as
bombesin o r lysophosphatidic acid (2-3). Phosph o r
ylation of FAK Tyr-397 c r e a t e s a binding site
f o r Src-family kinases, a n d phosph o r ylation of
FAK Tyr-576/Tyr-577 in the kinase domain activation
loop enhances catalytic activity (4). Increased
FAK expression has been c o r related with the
enhanced motility a n d invasiveness of human tum o r
cells, as well as with promoting increased
cell proliferation (5).
WB: 1:50,000 100,000
Flow Cytometry: 1:50
A. Western blot analysis on 3T3 cell lysates
using anti-Phospho-FAK (pY576) RabMAb
(cat. #2103-1), 1:50,000 dilution. Cells were
either (A) untreated (B) treated with pervanadate.
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