Rabbit Polyclonal Antibody

Molecular Formula：

Molecular Weight：

Alias：
KRT7 antibody, CK7 antibody, K2C7 antibody, K7 antibody, MGC129731 antibody, MGC3625 antibody, SCL antibody, Keratin, type II cytoskeletal 7 antibody, Sarcolectin antibody, Type-II keratin Kb7 antibody

Product order：

Name	Specifications	Packing	Price	Delivery
Rabbit Polyclonal Antibody		100ul	Inquiry	现货

Character：
【反应特异性】Human
【克隆 ID】polyclonal
【用途】WB , IHC , ICC , IP
【抗原分子量】55kDa

Quality Standard：
【产品介绍】Keratins are cytoplasmic intermediate
filament proteins expressed by epithelial cells.
Keratin 7 (K7) is expressed in a wide range of
epithelial structures in humans. The expression pattern
in mice is similar to the reported human distribution,
with substantial expression observed in lung,
bladder, mesothelium, hair follicle, and ductal
structures (1). Protein expression levels of CK7
appear significantly higher in chromophobe RCC compared
to oncocytoma (p < 0.001). Studies provide further
evidence that CK7 and parvalbumin immunostains may be
useful in differentiating oncocytoma from chromophobe RCC
in problematic cases (2). Also, the pattern
of immunohistochemical expression of CK 7 along with CK
20 is commonly used to assess possible primary sites
of metastatic carcinomas (3).

Recommended Applications

WB, IHC, ICC, IP
Applications and Recommended Dilution Factors

WB: 1:2000
IHC: 1:100 - 250
ICC: 1:100 - 250
IP: 1:30
Species Reactivity *

Human

*Cross reactivity determined by western blot only.

Products Data

A. Western blot analysis on T-47D cell lysate of
using anti-CK-7 Rabbit Polyclonal (cat. # 1899-1),
dilution 1:2000.

B. Immunohistochemical analysis of paraffin-embedded
human bladder cancer using anti-CK-7 Rabbit Polyclonal
(cat. #1899-1).

C. Immunofluorescent staining of HeLa cells using
anti-CK7 Rabbit Polyclonal antibody (cat. #1899-1).

Specificity

A synthetic peptide corresponding to residues near
the N-terminus of human CK-7 was used as an immunogen.

Storage：
Storage Condition and Buffer

Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4),
0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and
0.05% BSA. Stable for 12 months from date of receipt.