Rabbit Monoclonal Antibody

Molecular Formula：

Molecular Weight：

Alias：
CAMK2B antibody, CAM2 antibody, CAMK2 antibody, CAMKB antibody, MGC29528 antibody, Calcium/calmodulin-dependent protein kinase type II subunit beta antibody, antibody

Product order：

Name	Specifications	Packing	Price	Delivery
Rabbit Monoclonal Antibody		100ul	Inquiry	现货

Character：
【反应特异性】Human , Mouse , Rat
【克隆 ID】EPR6685(B)
【用途】WB , FC
【抗原分子量】45 - 70kDa

Quality Standard：
【产品介绍】Calcium/calmodulin-dependent protein kinase
II (CaMKII) is a widely distributed protein kinase
that regulates numerous physiological functions.
Inhibitors of CaMKII are useful tools for investigating
the CaMKII functions (1). The synapse contains
densely localized and interacting proteins that enable it
to adapt to changing inputs. A Ca2+-sensitive
protein complex is involved in the regulation of
AMPA receptor synaptic plasticity. The complex is
comprised of MUPPI, a multi-PDZ domain-containing
protein; SynGAP, a synaptic GTPase-activating protein;
and the Ca2+/calmodulin-dependent kinase CaMKII. In
synapses of hippocampal neurons, SynGAP and CaMKII
are brought together by direct physical interaction with
the PDZ domains of MUPP1, and in this complex, SynGAP
is phosphorylated. Ca2+CaM binding to CaMKII dissociates
it from the MUPP1 complex, and Ca2+ entering via the
NMDAR drives the dephosphorylation of SynGAP (2).

Recommended Applications

WB, FC
Applications and Recommended Dilution Factors

WB: 1:1,000 - 10,000
FC: 1:10 - 100
Species Reactivity *

Human, Mouse, Rat

*Species cross-reactivity is based on WB analysis.

Products Data

Western blot analysis on (A) human pancreas, (B)
fetal kidney, (C) HeLa, (D) Jurkat, (E) SH-SY5Y,
(F) BxPC-3, and (G) C6 lysates using anti-CaMKII
RabMAb (cat. #5475-1).

Flow cytometric analysis of permeabilized C6 cells
using anti-CaMKII RabMAb (red) (catalog # 5475-1) or
a rabbit IgG (negative) (green).

Specificity

A synthetic peptide corresponding to residues in
human CaMKII was used as an immunogen.

Storage Condition and Buffer

Store at -20° C. Buffer: Antibody buffer, sodium
azide, glycerol, and BSA. Stable for 12 months from date
of receipt.