Rabbit Monoclonal Antibody

Molecular Formula：

Molecular Weight：

Alias：
BTK,AGMX1, AT, ATK, BPK, IMD1, MGC126261, MGC126262, PSCTK1, XLA,Tyrosine-protein kinase BTK,Bruton tyrosine kinase

Product order：

Name	Specifications	Packing	Price	Delivery
Rabbit Monoclonal Antibody		100ul	Inquiry	现货

Character：
【反应特异性】Human , Mouse , Rat
【克隆 ID】EP420Y
【用途】WB , IP
【抗原分子量】77 kDa

Quality Standard：
【产品介绍】Btk (Bruton tyrosine kinase also known as BPK
or ATK) is a member of the TEC family of
non-receptor protein tyrosine kinase which includes Bmx,
Itk and TEC. Btk is composed of a pleckstrin domain, an
SH3 and SH2 domain, a catalytic kinase domain and a
TEC homology domain (1). Tec family protein tyrosine
kinases (TFKs) play a central role in hematopoietic
cellular signaling. Initial activation takes place
through specific tyrosine phosphorylation situated in
the activation loop. Further activation occurs within
the SH3 domain via a transphosphorylation mechanism,
which for Bruton's tyrosine kinase (Btk) affects
tyrosine 223. (2). Similar to Etk-TNFR2 interaction,
Btk interacts with Fas, a member of the TNFR family
involved in apoptosis in a ligand-independent manner
(3). Btk can be activated through
intramolecular autophosphorylation at tyrosine 551 and
that Btk autophosphorylation is required for Btk
catalytic functions (4).

Recommended Applications

WB, IP
Applications and Recommended Dilution Factors

WB: 1:10,000 - 50,000
IP: 1:40
Species Reactivity *

Human, Mouse, Rat

*Mouse and Rat reactivity predicted based on sequence alignment only, not based on testing

Products Data

A. Western blot analysis on Ramos cell lysates
using anti-Phospho-BTK (pY223) RabMAb (cat. #2207-1).
Cells were either (A) untreated (B) treated with
pervanadate.

Specificity

A phospho specific peptide corresponding to
residues surrounding tyrosine 223 of human BTK was used
as an immunogen. This antibody detects BTK phosphorylated
on tyrosine 223.

Storage：
Storage Condition and Buffer

Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4),
0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05%
BSA. Stable for 12 months from date of receipt.