Quality Standard：【产品介绍】JNK protein kinases are distantly related
to mitogen-activated protein kinases (ERKs) and
are activated by dual phosphorylation on Tyr and Thr.
The JNK protein kinase group includes the 46-kDa
isoform JNK1 and the 55-kDa protein kinase JNK2.
The activities of both JNK isoforms are markedly
increased by exposure of cells to UV radiation (1).
JNK becomes activated in vivo in response
to pro-inflammatory cytokines or cellular stresses. Its
full activation requires the phosphorylation of a
threonine and a tyrosine residue in a Thr-Pro-Tyr
motif, which can be catalysed by the protein kinases
mitogen-activated protein kinase kinase MKK4 and MKK7
(2). JNK2 functions in a cell-type-specific and
stimulus-dependent manner, being required for apoptosis
of immature thymocytes induced by anti-CD3 antibody but
not for apoptosis induced by anti-Fas antibody, UVC
or dexamethasone. JNK2 is not required for
activation-induced cell death of mature T cells (3).
Recommended Applications
WB, IHC, ICC, IP, FC
Applications and Recommended Dilution Factors
WB: 1:2,000 - 50,000
IHC: 1:100 - 250
ICC: 1:100 - 250
IP: 1:100
FC: 1:40
Species Reactivity *
Human, Mouse, Rat
*For Mouse, this antibody is recommended for WB only
Rat cross reactivity tested by western blot only
Products Data
A. Western blot analysis on HeLa cell lysate using
anti-JNK2 RabMAb (cat. # 2037-1), dilution 1:50,000.
B. Immunohistochemical staining of paraffin-embedded
human breast carcinoma using anti-JNK2 RabMAb (cat. #2037-1).
Specificity
A synthetic peptide corresponding to residues near
the C-terminus of human JNK2 was used as an immunogen.
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