Quality Standard：【产品介绍】Erzin, a member of the Ezrin, Radixin,
Moesin (ERM) family, is a linker protein located
between cell surface recept o r s, adhesion molecules,
a n d actin cytoskeleton (1-2). Ezrin activity is
regulated by intramolecular interactions between
N- a n d C-terminal ERM association domains (3).
Erzin tyrosine phosph o r ylation can also be induced
by EGF, PDGF a n d HGF stimulation. Erzin interacts
with PI3-K protein kinase A a n d Rho (4). In response
to epidermal growth fact o r (EGF) the microvillar
c o r e protein ezrin is phosph o r ylated transiently to
a high level on tyrosine residues in human
epidermoid carcinoma A431 cells. Tyr145, lies in
the N-terminal region of homology that is common to the
b a n d 4.1-talin-ezrin protein family. This
tyrosine residue a n d its vicinal amino acids are
conserved throughout the family members, including
radixin, moesin, a n d the two phosphotyrosine
phosphatases, PTP H1 a n d PTP MEG, but not in b a n d 4.1
o r talin (5).
WB: 1:1000 - 5000
ICC: 1:100 – 250
Flow Cytometry: 1:20
A. Western blot analysis on (A) 293, (B) A431
a n d (C) SH-SY5Y cell lysate using anti-Ezrin RabMAb
(cat. # 2255-1), dilution 1:10,000.
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