Rabbit Monoclonal Antibody

Molecular Formula：

Molecular Weight：

Alias：
KRT20 antibody, CD20 antibody, CK-20 antibody, CK20 antibody, K20 antibody, KRT21 antibody, MGC35423 antibody, Keratin, type I cytoskeletal 20 antibody, Protein IT antibody

Product order：

Name	Specifications	Packing	Price	Delivery
Rabbit Monoclonal Antibody		100ul	Inquiry	现货

Character：
【反应特异性】Human , Mouse , Rat
【克隆 ID】EPR1622Y
【用途】WB , IHC , ICC , IP
【抗原分子量】46kDa

Quality Standard：
【产品介绍】Intermediate-sized filament (IF)
protein designated cytokeratin (CK) 20 is a major
cellular protein of mature enterocytes and goblet
cells commonly found in mucosal epithelium of the
mammalian gastrointestinal tract (1). Results
strongly suggest that transcriptional regulation of
keratin genes in the intestinal epithelium occurs at
the level of both immature and terminally
differentiated epithelial cells, and is tightly
regulated during both fetal development and
crypt-to-villus differentiation of the intestinal
epithelium (2). CK20 has recently been reported to be
useful to distinguish between primary and metastatic
lung adenocarcinoma. CK20 expression was significantly
more prevalent in adenocarcinoma that originated in the
GI tract than that of pulmonary or breast origin (3).

Recommended Applications

WB, IHC, ICC, IP
Applications and Recommended Dilution Factors

WB: 1:10,000 - 50,000
IHC: 1:100 - 250
ICC: 1:100 - 250
IP: 1:40
Species Reactivity *

Human, Mouse, Rat

*Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

Products Data

A. Western blot analysis on human small intestine
lysate using anti-CK20 RabMAb (cat. # 2039-1),
dilution 1:50,000.

B. Immunohistochemical analysis of paraffin-embedded
human colon adenocarcinoma using anti-CK-20 RabMAb
(cat. #2039-1).

Specificity

A synthetic peptide corresponding to residues on
the C-terminus of human Cytokeratin 20 was used as
an immunogen.

Storage：
Storage Condition and Buffer

Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4),
0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and
0.05% BSA. Stable for 12 months from date of receipt.