Tris(hydroxymethyl)aminomethane hydrochloride

Molecular Structure：

MDL：
MFCD00012590

Molecular Formula：
C4H12CINO3；NH2C(CH2OH)3·HCl

Molecular Weight：
157.60

Alias：
三-(羟甲基)氨基甲烷盐酸 TRIS HCl TRIS hydrochloride Tris(hydroxymethyl)aminomethane hydrochloride

Product order：

Name	Specifications	Packing	Price	Delivery
Tris(hydroxymethyl)aminomethane hydrochloride	高纯级,>99.5% (滴定)	100g	RMB 264	现货
Tris(hydroxymethyl)aminomethane hydrochloride	高纯级,>99.5% (滴定)	500g	RMB 1122	现货
Tris(hydroxymethyl)aminomethane hydrochloride	用于分子生物学和细胞培养，≥99.0% (AT)	500g	RMB 1485	Consulting service
Tris(hydroxymethyl)aminomethane hydrochloride	用于分子生物学和细胞培养，≥99.0% (AT)	100g	RMB 616	Consulting service
Tris(hydroxymethyl)aminomethane hydrochloride	超纯级	500g	RMB 968	Consulting service
Tris(hydroxymethyl)aminomethane hydrochloride	超纯级	100g	RMB 220	Consulting service
Tris(hydroxymethyl)aminomethane hydrochloride	超纯级	25g	RMB 88	现货
Tris(hydroxymethyl)aminomethane hydrochloride	99%	500g	RMB 418	Consulting service
Tris(hydroxymethyl)aminomethane hydrochloride	99%	100g	RMB 88	Consulting service

Character：
溶于水
熔点：150-152°C
pKa (25 °C) 8.1

Quality Standard：
项目                                 生化试剂级
                                        BC
纯度assay                             ≥99%(T)
水分Moistrue                          ≤0.5%
重金属Heavy Metals                    <5PPM
pH(1% in H2O)                         4.7-6.0

项目                                  超纯级
纯度assay                             ≥99.0%(T)
水分Moistrue                          ≤0.5%
铁Iron(Fe)                            <5PPM
氯Chloride                            ≤0.005%
硫酸根Sulfate                         ≤0.005%
重金属Heavy Metals （as Pb)           <5PPM
灼烧残渣Residue On Ignition           ≤0.1%
A260(1M,water):                       ≤0.025
A280(1M,water):                       ≤0.020

品级Grade                               分子生物学级
                                  (grade for molecular biology)
含量assay                             ≥99.0% (AT)
DNA酶DNases,                          none detected
RNA酶RNases,                          none detected
不溶物insoluble matter,               passes filter test
磷酸酶phosphatases,                   none detected
蛋白酶proteases,                      none detected
细胞培养测试cell culture test         Pass
灼烧残渣ign. residue                  ≤0.2% (as SO4)
干燥损失loss on drying                ≤0.2% , 110 °C
pH                                    2.5-4.0 (25 °C, 4 M in H2O)
useful pH range                       7.0 - 9.0
pKa (25 °C)                          8.1
水溶性solubility H2O:                 soluble4 M at 20 °C, clear, colorless
硫酸根sulfate (SO42-):                ≤50 ppm
  Al:                                 ≤5 ppm
  As:                                 ≤0.1 ppm
  Ba:                                 ≤5 ppm
  Bi:                                 ≤5 ppm
  Ca:                                 ≤10 ppm
  Cd:                                 ≤5 ppm
  Co:                                 ≤5 ppm
  Cr:                                 ≤5 ppm
  Cu:                                 ≤5 ppm
  Fe:                                 ≤5 ppm
  K:                                 ≤50 ppm
  Li:                                 ≤5 ppm
  Mg:                                 ≤5 ppm
  Mn:                                 ≤5 ppm
  Mo:                                 ≤5 ppm
  Na:                                 ≤50 ppm
  Ni:                                 ≤5 ppm
  Pb:                                 ≤5 ppm
  Sr:                                 ≤5 ppm
  Zn:                                 ≤5 ppm
λ 4 M in H2O
UV absorption
290nm A                              ≤0.05 AT (40% W/W)
260nm A                              ≤0.06 AT (1M IN WATER)
280nm A                              ≤0.06 AT (1M IN WATER

Storage：
室温保存
用途所有缓冲液的pH值依赖于温度和浓度。对于Tris 缓冲液,温度每增加1°C pH增加大约 0.03 单位, 每10倍的稀释pH减少0.03-0.05 单位。