质量标准：【产品介绍】Chk1 is a serine/threonine-protein
kinase required for checkpoint mediated cell cycle arrest
in response to DNA damage or the presence of
unreplicated DNA (1). It is regulated by Atr and plays
an important role in embryonic development and
tumor suppression (2). The activated Chk1 triggers the
G2/M checkpoint, which arrests the cells in G2 to allow
them to repair their DNA (3). The inhibition of Chk1
kinase results in the abrogation of the G2/M checkpoint
and leads to cell death (3). Chk1 is activated in
response to replication blocks and genotoxic stress,
via phosphorylation of serines 317 and 345 (4).
Activated Chk1 can bind to, phosphorylate
(at serine 216), and inactivate cdc25c (4, 5).
Recommended Applications
WB
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
Species Reactivity
Human
Products Data
Western blot analysis on (A) Jurkat (B) K562, (C) MCF-7,
and (D) HepG52 cell lysates using anti-Chk1 RabMAb
(cat. # 2865-1).
Specificity
A synthetic peptide corresponding to residues in human
Chk1 was used as an immunogen.
贮存：Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4),
0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and
0.05% BSA. Stable for 12 months from date of receipt.
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