Quality Standard：【产品介绍】Oncogene-encoded proteins c-Myc, N-Myc,
and L-Myc function in cell proliferation,
differentiation and neoplastic disease. Amplification of
the c-Myc gene has been found in several types of
human tumors, the N-Myc gene in neuroblastomas (1), and
the L-Myc gene in human small cell lung carcinomas
(2). c-Myc protein is a transcription factor localized
to the nucleus of the cell. It seems to be involved
in activating the transcription of growth-related
genes. c-Myc binds to DNA during transcription as
a heterodimeric complex with Max. c-Myc is phosphorylated
in vitro by p44/42 MAP kinase at Ser62 (3) and in vivo
at both Thr58 and Ser62. Mutation of Thr58 and Ser62 to
Ala inhibits the ability of c-Myc to activate
transcription (3).
Recommended Applications
ICC
Applications and Recommended Dilution Factors
ICC: 1:50 - 100
Species Reactivity
Human
Products Data
Immunofluorescent staining of HeLa cells using
anti-c-Myc antibody conjugated with Dylight 488
(green, Cat# 4581-1) and Dapi (blue).
Specificity
A synthetic peptide corresponding to residues in
N-terminus of human c-Myc was used as immunogen.
Storage：Storage Condition and Buffer
Store at 2-8° C. PBS (pH 7.2), 0.02% sodium azide,
0.05% BSA. Protect from light. Stable for 6 months from
date of receipt.
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