质量标准:【产品介绍】The Beclin-1 (BECN1) gene encodes a
60-kDa coiled-coil protein that interacts with
the prototypic apoptosis inhibit o r Bcl-2 (1).
Beclin-1 promotes autophagy in autophagy-defective
yeast with a targeted disruption of agp6/vps30, a n d
in human MCF7 breast carcinoma cells. The
autophagy-promoting activity of Beclin-1 in MCF7 cells
is associated with inhibition of MCF7 cellular proliferation, in vitro clonigenicity a n d tum
o r igenesis. Endogenous Beclin-1 protein expression
is frequently low in human breast epithelial carcinoma
cell lines a n d tissue, but is expressed ubiquitously
at high levels in n o r mal breast epithelia (2). It
has also been shown that Beclin 1 contains a
leucine-rich nuclear exp o r t signal motif raising
the possibility that its autophagy a n d / o r tum
o r suppress o r function may require
regulated, CRM1-dependent, nucleocytoplasmic
transp o r t (3).
WB: 1:5000
IHC: 1:50
ICC: 1:50 100
IP: 1:50
A. Western blot analysis on A431 cell lysate
using anti-Beclin-1 RabMAb (cat. # 2026-1), dilution 1:5000.
B. Immunohistochemical staining of paraffin-embedded
human breast tissue using anti-Beclin-1 RabMAb (cat. #2026-1).