IL10

Molecular Formula：

Molecular Weight：

Alias：
IL10 antibody, CSIF antibody, IL-10 antibody, IL10A antibody, MGC126450 antibody, MGC126451 antibody, TGIF antibody, Interleukin-10 antibody, Cytokine synthesis inhibitory factor antibody

Product order：

Name	Specifications	Packing	Price	Delivery
IL10		100ul	Inquiry	现货

Character：
【产品类型】Rabbit Monoclonal Antibody
【反应特异性】Human
【克隆 ID】EPR1114
【用途】WB , ICC , FC , IP
【产品性状】
【阳性对照】
【产品浓度】
【细胞定位】
【抗原分子量】18kDa

Quality Standard：
【产品介绍】Interleukin (IL)-10 is a cytokine that
inhibits production of other regulatory factors,
including interferon gamma (IFN-gamma) and IL-2. A dimer
of IL-10 is present in solution and is presumed
to participate in receptor binding (1). IL-10 is a
natural endogenous immunosuppressive cytokine that has
been identified in human, murine, and other organisms.
Human IL-10 (hIL-10) has high homology with murine
IL-10 (mIL-10) as well as with an Epstein-Barr virus
genome product BCRFI (2). IL-10 displays
pleiotropic immunoregulatory properties. Human and
murine IL-10 have a high amino acid sequence identity
(ca. 73%) which includes the conservation of all
four cysteine residues in human IL-10 and the first four
out of five cysteine residues for murine IL-10. IL-10 is
a highly alpha-helical protein containing two
disulfide bonds, either one or both of which are
critical for its structure and function (3).

Recommended Applications
WB, ICC, IP, FC
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
ICC: 1:50 - 100
IP: 1:10 - 100
FC: 1:100 - 500
Species Reactivity *
Human
*Species cross-reactivity is based on WB analysis.

Products Data

Western blot analysis on (A) THP-1 treated with LPS,
(B) THP-1, and (C) Ramos cell lysates using
anti-IL-10 RabMAb (cat. #5339-1).

Western blot analysis on 5 ng of IL-10 recombinant
protein using anti-IL-10 RabMAb (cat. #5339-1).

Flow cytometric analysis of permeabilized HeLa cells
using anti-IL-10 RabMAb (red) (catalog # 5339-1) or a
rabbit IgG (negative) (green).

Specificity
A synthetic peptide corresponding to residues in human
IL-10 was used as an immunogen.

Storage：
Store at -20 °C. Antibody buffer, 0.01% sodium azide,
50% Glycerol and 0.05% BSA. Stable for 12 months from date
of receipt