Quality Standard：【产品介绍】Calcium/calmodulin-dependent protein kinase
II (CaMKII) is a widely distributed protein kinase
that regulates numerous physiological functions. It is
a ubiquitous serine/threonine protein kinase which
is abundant in brain as a major PSD constituent. This
enzyme is composed of distinct but related subunits
(alpha, beta, gamma, and delta) (1). The synapse
contains densely localized and interacting proteins
that enable it to adapt to changing inputs. A
Ca2+-sensitive protein complex involved in the regulation
of AMPA receptor synaptic plasticity. The complex
is comprised of MUPPI, a multi-PDZ
domain-containing protein; SynGAP, a synaptic
GTPase-activating protein; and the
Ca2+/calmodulin-dependent kinase CaMKII. In synapses
of hippocampal neurons, SynGAP and CaMKII are
brought together by direct physical interaction with the
PDZ domains of MUPP1, and in this complex, SynGAP
is phosphorylated. Ca2+CaM binding to CaMKII dissociates
it from the MUPP1 complex, and Ca2+ entering via the
NMDAR drives the dephosphorylation of SynGAP (2). The
most important autophosphorylation site is Threonine
286, which resides in the vicinity of the
autoinhibitory domain (3).
Recommended Applications
WB
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
Species Reactivity *
Human, Mouse, Rat
*Species cross-reactivity is based on WB analysis
Products Data
Western blot analysis on rat brain lysates
using anti-Phospho-CaMKII (pT286) RabMAb
(cat. #3891-1). Cells were either (A) untreated (B)
treated with Lambda Phosphatase.
Specificity
A phospho specific peptide corresponding to
residues surrounding Threonine 286 of human CaMKII was
used as an immunogen. This antibody only detects
CaMKII phosphorylated at Threonine 286.
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